Manual of Human Growth Factor (HGH) Enzyme Linked Immunoassay (ELISA)
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Human growth factor (HGH) enzyme-linked immunoassay (ELISA)
Kit instruction manual
This reagent is for research use only
Purpose: This kit is used to determine the content of growth factor (HGH) in human serum, plasma and related liquid samples.
Experimental principle:
This kit uses the double antibody sandwich method to determine the level of human growth factor (HGH) in the specimen. The microplate was coated with purified human growth factor (HGH) antibody to make a solid phase antibody. Growth factors (HGH) were added to the monoclonal antibody-coated microwells in turn, and then combined with HRP-labeled growth factor (HGH) antibody To form an antibody-antigen-enzyme-labeled antibody complex, and after thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the growth factor (HGH) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human growth factor (HGH) in the sample was calculated by a standard curve.
Precautions:
1. Crystals may be precipitated in the concentrated washing liquid, which can be heated and dissolved in a water bath during dilution, and the results will not be affected during washing.
2. The sealing film is limited to one-time use to avoid cross-contamination.
3. The sampler should be used at each step of sample addition, and the accuracy should be regularly checked to avoid test errors. It is best to control the sampling time within 5 minutes. If there are many specimens, it is recommended to use a volley gun to add samples.
4. The kit should be equilibrated at room temperature for 15-30 minutes before being taken out of the refrigerated environment. If the enzyme label coated plate is unopened, the strip should be stored in a sealed bag.
5. Please make a standard curve at the same time of each measurement, it is best to make a double hole. If the content of the test substance in the specimen is too high (the OD value of the sample is greater than the OD value of the first well of the standard well), please first dilute it with a certain multiple (n times) of the sample diluent and then determine it. When calculating, please multiply the total dilution Multiple (× n × 5).
6. Please keep the substrate away from light.
7. Strictly follow the instructions, and the test results must be determined by the microplate reader.
8. All samples, washing liquids and various wastes should be treated as infectious agents.
9. The components of different batches of this reagent shall not be mixed.
10. If there is any difference with the English manual, the English manual shall prevail.
